Plasmid_Backbone

Part:BBa_K4216046:Design

Designed by: Yves Loiseau--Marchand   Group: iGEM22_Paris_Bettencourt   (2022-10-11)


V35m


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found at 2772
    Illegal suffix found at 800
    Illegal SpeI site found at 2794
    Illegal SpeI site found at 2838
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2772
    Illegal NheI site found at 2863
    Illegal NheI site found at 2886
    Illegal SpeI site found at 801
    Illegal SpeI site found at 2794
    Illegal SpeI site found at 2838
    Illegal PstI site found at 815
    Illegal NotI site found at 808
    Illegal NotI site found at 2778
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2772
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 2772
    Illegal suffix found at 801
    Illegal SpeI site found at 2794
    Illegal SpeI site found at 2838
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 2772
    Plasmid lacks a suffix.
    Illegal XbaI site found at 2787
    Illegal SpeI site found at 801
    Illegal SpeI site found at 2794
    Illegal SpeI site found at 2838
    Illegal PstI site found at 815
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 781
    Illegal BsaI.rc site found at 2814


Design Notes

N/A


Source

CIDAR moclo

References